Initiation of eukaryotic messenger RNA synthesis.
نویسندگان
چکیده
منابع مشابه
Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA.
A heterogeneous RNA fraction with properties resembling those of messenger RNA was identified in mammalian mitochondria. Synthesis of contaminating RNA of nuclear origin was suppressed by treatment with camptothecin. Labeling of the messenger-like RNA is completely inhibited by ethidium bromide, a specific inhibitor of mitochondrial functions.Although mitochondrial protein synthesis resembles t...
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The ability of polypeptide components of eukaryotic initiation factor (eIF) 4F to bind to the m7G cap of an mRNA, to be released from that mRNA, and then to rebind to the cap of a second mRNA has been investigated. The release and rebinding steps have been termed "recycling." It was found that eIF-4B stimulates the recycling of the 24-26 kDa (p24) component of eIF-4F, and perhaps of other compo...
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Interaction of protein synthesis initiation factors with mRNA has been studied in order to characterize early events in the eukaryotic translation pathway. Individual reovirus mRNAs labeled with 32P in the alpha position relative to the m7G cap and eukaryotic initiation factor (eIF)-4A, -4B, and -4F purified from rabbit reticulocytes were employed. It was found that eIF-4A causes a structural c...
متن کاملMolecular design of a eukaryotic messenger RNA and its chemical synthesis.
A designed mRNA consisting of 42 ribonucleotides having the cap structure was synthesized. The capped leader sequence of the brome mosaic virus (BMV) mRNA 4, m7G5'pppGUAUUAAUA (F-1), was synthesized by the phosphotriester method and followed by the capping reaction. A 32-mer consisting of an initiation codon (AUG), the coding region corresponding to a bacterial pheromone cAD1 and two stop codon...
متن کاملInterference of nonsense mutations with eukaryotic messenger RNA stability.
The fine structure map of the yeast URA 3 gene was established by meiotic recombination, and amber nonsense mutations were located at different points on the map. The effect of the length of the labeling time on the specific radioactivity of ura 3 messenger RNA and on its repartition between poly(A)-RNA and RNA not containing poly(A) has been followed in nonsense mutants. Nonsense mutations red...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55182-6